Evaluation of a solid phase radioimmunoassay (Ausria-125) for the detection of hepatitis B antigen (Australia antigen) in two clinical materials

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Evaluation of a solid phase radioimmunoassay (Ausria-125) for the detection of hepatitis B antigen (Australia antigen) in two clinical materials.

A solid-phase radioimmunoassay (Ausria-125) was compared to an immunoelectro-osmophoresis method in detecting hepatitis-B antigen (HBAg). The radioimmunoassay detected HBAg in approximately 230 times higher dilutions than the immunoelectroosmophoresis method. In cases of acute hepatitis HBAg could be detected for about 70% longer after the onset of jaundice using radioimmunoassay compared with ...

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Detection of Australia antigen by radioimmunoassay.

A double-antibody radioimmunoassay has been developed for the detection of Australia antigen and antibodies to Australia antigen in human sera. The assay is simple to perform, and can be completed in 18 hr. Based on parallel studies of human sera, it is approximately 2000 times more sensitive than agar gel diffusion and 200 times more sensitive than quantitative complement fixation. Australia a...

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Serum hepatitis B core antigen (HBcAg) is an important marker of hepatitis B virus replication. We describe an easy, sensitive radioimmunoassay for determination of HBcAg in detergent-treated serum pellets containing Dane particles. Components of a commercial kits for anti-core determination are used, and HBcAG is measured by competitive inhibition of binding of 125I-labeled antibodies to HBcAg...

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EXPRESSION OF HEPATITIS B SURFACE ANTIGEN IN SACCHAROMYCES CEREVISIAE

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1974

ISSN: 0021-9746

DOI: 10.1136/jcp.27.1.35